[4-氯-D-苯丙氨酸6，亮氨酸17]血管活性肠肽对去卵巢大鼠中血管活性肠肽及相关肽抑制促黄体生成素脉冲式释放的影响。

Effect of [4Cl-D-Phe6,Leu17]VIP on the inhibition of pulsatile LH release by VIP and related peptides in the ovariectomized rat.

作者信息

Weick R F, Stobie K M, Noh K A

机构信息

Department of Physiology, University of Western Ontario, London, Canada.

出版信息

Neuroendocrinology. 1992 Nov;56(5):646-52. doi: 10.1159/000126288.

DOI:10.1159/000126288

PMID:1488097

Abstract

Pulsatile LH secretion in the ovariectomized (OVX) rat is inhibited by intracerebroventricular (icv) infusion of vasoactive intestinal peptide (VIP). VIP, rat growth hormone-releasing hormone (rGRH) and secretin with and without an antagonist to VIP, [4Cl-D-Phe6,Leu17]VIP (VIPA), were infused icv into OVX rats. Both VIP and rGRH at an infusion rate of 3.5 nmol/h lowered mean LH concentrations and pulse frequency without affecting pulse amplitude, and these effects were blocked by concurrent infusion of VIPA (10.5 nmol/h). Secretin also inhibited pulsatile LH secretion, but was only fully effective at the higher infusion rate of 7 nmol/h. This effect of secretin was also blocked by concurrent infusion of 10.5 nmol/h of VIPA. These results suggest that all three of these peptide hormones inhibit pulsatile LH secretion by an interaction with VIP receptors.

摘要

在去卵巢（OVX）大鼠中，脑室内（icv）注入血管活性肠肽（VIP）可抑制促黄体生成素（LH）的脉冲式分泌。将VIP、大鼠生长激素释放激素（rGRH）和促胰液素分别或与VIP拮抗剂[4Cl-D-Phe6,Leu17]VIP（VIPA）一起icv注入OVX大鼠体内。以3.5 nmol/h的输注速率注入VIP和rGRH均可降低LH的平均浓度和脉冲频率，且不影响脉冲幅度，同时注入VIPA（10.5 nmol/h）可阻断这些作用。促胰液素也可抑制LH的脉冲式分泌，但仅在7 nmol/h的较高输注速率下才完全有效。同时注入10.5 nmol/h的VIPA也可阻断促胰液素的这种作用。这些结果表明，这三种肽类激素均通过与VIP受体相互作用来抑制LH的脉冲式分泌。