Weick R F, Stobie K M, Noh K A
Department of Physiology, University of Western Ontario, London, Canada.
Neuroendocrinology. 1992 Nov;56(5):646-52. doi: 10.1159/000126288.
Pulsatile LH secretion in the ovariectomized (OVX) rat is inhibited by intracerebroventricular (icv) infusion of vasoactive intestinal peptide (VIP). VIP, rat growth hormone-releasing hormone (rGRH) and secretin with and without an antagonist to VIP, [4Cl-D-Phe6,Leu17]VIP (VIPA), were infused icv into OVX rats. Both VIP and rGRH at an infusion rate of 3.5 nmol/h lowered mean LH concentrations and pulse frequency without affecting pulse amplitude, and these effects were blocked by concurrent infusion of VIPA (10.5 nmol/h). Secretin also inhibited pulsatile LH secretion, but was only fully effective at the higher infusion rate of 7 nmol/h. This effect of secretin was also blocked by concurrent infusion of 10.5 nmol/h of VIPA. These results suggest that all three of these peptide hormones inhibit pulsatile LH secretion by an interaction with VIP receptors.
在去卵巢（OVX）大鼠中，脑室内（icv）注入血管活性肠肽（VIP）可抑制促黄体生成素（LH）的脉冲式分泌。将VIP、大鼠生长激素释放激素（rGRH）和促胰液素分别或与VIP拮抗剂[4Cl-D-Phe6,Leu17]VIP（VIPA）一起icv注入OVX大鼠体内。以3.5 nmol/h的输注速率注入VIP和rGRH均可降低LH的平均浓度和脉冲频率，且不影响脉冲幅度，同时注入VIPA（10.5 nmol/h）可阻断这些作用。促胰液素也可抑制LH的脉冲式分泌，但仅在7 nmol/h的较高输注速率下才完全有效。同时注入10.5 nmol/h的VIPA也可阻断促胰液素的这种作用。这些结果表明，这三种肽类激素均通过与VIP受体相互作用来抑制LH的脉冲式分泌。
