利用针对班氏吴策线虫微丝蚴SDS可溶性抗原产生的抗体检测丝虫抗原。

Detection of filarial antigen using antibodies raised against Wuchereria bancrofti microfilarial SDS soluble antigen.

作者信息

Cheirmaraj K, Reddy M V, Harinath B C

机构信息

Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, India.

出版信息

Southeast Asian J Trop Med Public Health. 1992 Sep;23(3):444-9.

PMID:1488700

Abstract

Polyclonal antibodies raised in mouse ascitic fluid against Wuchereria bancrofti microfilarial antigens (Wb Mf SDS S Ag) were studied for their diagnostic use in bancroftian filariasis using a dip stick, enzyme-linked immunosorbent assay. In sandwich ELISA, 100% of microfilaremic sera (30 out of 30) 53% of acute filarial sera (7/13), 40% of subacute filarial sera (6 out of 15), 13% of chronic filarial sera (2/15) and 20% of endemic area normal sera (3/15) showed the presence of filarial antigen. Determination of filarial antigen titer in microfilaremic sera showed an apparent positive correlation between microfilarial density and antigen titer. The antibody raised against Wb Mf SDS S Ag was found to be cross reactive with phosphorylcholine epitopes. The filarial antigen detected by anti Wb Mf SDS S Ag antibodies in sandwich ELISA is possibly associated with the active stage (microfilaremia) of infection.

摘要

研究了用抗班氏吴策线虫微丝蚴抗原（Wb Mf SDS S Ag）的小鼠腹水制备的多克隆抗体，通过试纸条、酶联免疫吸附测定法在班氏丝虫病诊断中的应用。在夹心酶联免疫吸附测定中，100%的微丝蚴血症血清（30份中的30份）、53%的急性丝虫病血清（13份中的7份）、40%的亚急性丝虫病血清（15份中的6份）、13%的慢性丝虫病血清（15份中的2份）和20%的流行区正常血清（15份中的3份）显示存在丝虫抗原。微丝蚴血症血清中丝虫抗原滴度的测定表明，微丝蚴密度与抗原滴度之间存在明显的正相关。发现抗Wb Mf SDS S Ag产生的抗体与磷酸胆碱表位有交叉反应。夹心酶联免疫吸附测定中抗Wb Mf SDS S Ag抗体检测到的丝虫抗原可能与感染的活跃期（微丝蚴血症）有关。