Singh T A, Cheirmaraj K, Reddy M V, Narang R, Harinath B C
Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Wardha, India.
Indian J Exp Biol. 1993 Jan;31(1):1-4.
Paired samples of serum and hydrocele fluid of filarial patients associated with hydrocele were analysed for filarial antibody and antigen. Sera samples showed higher titers of filarial antibody and antigen compared to their corresponding hydrocele fluid samples. HFIgG isolated from hydrocele fluid was equally useful as FSIgG isolated from serum and detected filarial antigen in 23 out of 26 microfilaraemic sera, 7 out of 10 chronic sera and 3 out of 18 endemic normal sera by inhibition ELISA. Filarial antigen was isolated from hydrocele fluid. In inhibition ELISA antigen fraction, HFA-9 (20-25 kDa) isolated by sodium-dodecyl sulphate-polyacrylamide gel electrophoresis showed high reciprocal antigen titer of 2048. This active antigen fraction was evaluated for its diagnostic utility in comparison with Wuchereria bancrofti microfilariae excretory-secretory antigen (Wb mf ES Ag) in inhibition ELISA. Both antigen preparations detected filarial antigen in about 80% of microfilaraemic sera, 60% chronic sera and 20-30% of endemic normal sera. This study showed that antibody and antigen isolated from hydrocele fluid were equally sensitive as FSIgG and Wb mf ES Ag in the detection of filarial antigen by inhibition ELISA. Thus hydrocele fluid may be used as an alternative source for the isolation of antibody and antigen of immunodiagnostic importance.
对伴有鞘膜积液的丝虫病患者的血清和鞘膜积液配对样本进行丝虫抗体和抗原分析。血清样本显示，与相应的鞘膜积液样本相比，丝虫抗体和抗原滴度更高。从鞘膜积液中分离出的HFIgG与从血清中分离出的FSIgG同样有用，通过抑制ELISA法，在26份微丝蚴血症血清中的23份、10份慢性血清中的7份以及18份地方性正常血清中的3份中检测到丝虫抗原。从鞘膜积液中分离出丝虫抗原。在抑制ELISA抗原组分中，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离出的HFA-9（20-25 kDa）显示出2048的高反向抗原滴度。在抑制ELISA中，将该活性抗原组分与班氏吴策线虫微丝蚴排泄分泌抗原（Wb mf ES Ag）比较，评估其诊断效用。两种抗原制剂在约80%的微丝蚴血症血清、60%的慢性血清以及20%-30%的地方性正常血清中检测到丝虫抗原。本研究表明，从鞘膜积液中分离出的抗体和抗原在通过抑制ELISA检测丝虫抗原方面与FSIgG和Wb mf ES Ag同样敏感。因此，鞘膜积液可作为分离具有免疫诊断重要性的抗体和抗原的替代来源。
