培养箱类型对体外生产牛囊胚产量的影响。
Impact of incubator type on the yield of in vitro produced bovine blastocysts.
作者信息
Avery B, Greve T
机构信息
Department of Clinical Studies, Reproduction, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
出版信息
Acta Vet Scand. 1992;33(4):341-8. doi: 10.1186/BF03547300.
Because of suboptimal in vitro production of bovine blastocysts a new incubator model (Mini) was tested against the traditional (Heraeus). The difference between their properties seemed only to be the volume of the incubator space. No difference was noted between the CO2 or the temperature, but the data clearly showed a highly significant increase of the blastocyst rates, 6% versus 51% in the Heraeus and the Mini incubator, respectively, calculated as blastocysts per cleaved embryos. It was concluded that the incubator type or model may be a very important part of the in vitro production of bovine embryos, although we were not able to pin point specific causes for this difference.
由于牛囊胚的体外生产效果欠佳，一种新型培养箱模型（Mini）与传统培养箱（Heraeus）进行了对比测试。它们在性能上的差异似乎仅体现在培养箱空间的容积上。二氧化碳浓度或温度方面未发现差异，但数据清楚地显示，囊胚率有显著提高，以每个分裂胚胎中的囊胚数计算，Heraeus培养箱和Mini培养箱的囊胚率分别为6%和51%。得出的结论是，培养箱类型或模型可能是牛胚胎体外生产中非常重要的一部分，尽管我们无法确定造成这种差异的具体原因。
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