一些影响输卵管组织条件培养液对早期牛胚胎培养效果的因素。

Some factors affecting the efficacy of oviduct tissue-conditioned medium for the culture of early bovine embryos.

作者信息

Eyestone W H, Jones J M, First N L

机构信息

Department of Meat and Animal Science, University of Wisconsin, Madison 53706.

出版信息

J Reprod Fertil. 1991 May;92(1):59-64. doi: 10.1530/jrf.0.0920059.

DOI:10.1530/jrf.0.0920059

PMID:2056496

Abstract

Oviduct tissue-conditioned medium was evaluated for the culture of IVM-IVF bovine zygotes to the compact morula (cM) and blastocyst (BL) stages. Development was unaffected (P greater than 0.50) by freezing and thawing of conditioned medium: no. cM + BL/no. cleaved ova obtained after culture in nonfrozen, frozen-thawed, and control treatments were 31/148 (21%), 26/124 (21%) and 5/86 (6%), respectively. The greatest proportion of normal development was obtained after a conditioning period of 48 h (P less than 0.05): no. of cM + BL/no. cleaved ova in media conditioned for 5, 24, 48 and 96 h were 23/114 (20%), 38/112 (34%), 43/115 (37%) and 36/125 (29%), respectively. Development declined with increasing dilutions of conditioned media (P less than 0.005): no. cM + BL/no. cleaved ova for 100, 75, 50, 25 and 0% conditioned medium were 32/94 (34%), 29/94 (31%), 17/82 (21%), 10/94 (11%) and 11/73 (15%), respectively. The oestrous cycle stage from which oviducal tissue was obtained did not affect development (P greater than 0.75); no. cM + BL/no. cleaved ova was 21/63 (33%) at oestrus and 21/79 (27%) in the luteal phase.

摘要

评估了输卵管组织条件培养基对体外成熟-体外受精牛受精卵培养至致密桑椹胚（cM）和囊胚（BL）阶段的效果。条件培养基的冻融对发育没有影响（P大于0.50）：在未冷冻、冻融和对照处理后培养获得的致密桑椹胚 + 囊胚数/分裂卵数分别为31/148（21%）、26/124（21%）和5/86（6%）。在48小时的条件培养期后获得的正常发育比例最高（P小于0.05）：在条件培养5、24、48和96小时的培养基中，致密桑椹胚 + 囊胚数/分裂卵数分别为23/114（20%）、38/112（34%）、43/115（37%）和36/125（29%）。随着条件培养基稀释度的增加，发育率下降（P小于0.005）：100%、75%、50%、25%和0%条件培养基的致密桑椹胚 + 囊胚数/分裂卵数分别为32/94（34%）、29/94（31%）、17/82（21%）、10/94（11%）和11/73（15%）。获取输卵管组织的发情周期阶段对发育没有影响（P大于0.75）；发情期的致密桑椹胚 + 囊胚数/分裂卵数为21/63（33%），黄体期为21/79（27%）。