Fawell S E, McKenzie M A, Greenfield N J, Adebodun F, Jordan F, Lenard J
Department of Physiology and Biophysics, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway 08854-5635.
Endocrinology. 1988 Feb;122(2):518-23. doi: 10.1210/endo-122-2-518.
Addition of bovine insulin to cells of the wall-less variant FGSC4761 of Neurospora crassa ("slime") produced several significant effects on glycogen metabolism. 1) Intracellular levels of the glycogen precursor UDP-glucose decreased 17-18% (P less than 0.01) within 30 min of insulin addition. 2) Cells grown with insulin possessed 40% more glycogen than did control cells. 3) The incorporation of 14C-labeled glucose into glycogen increased 41% after 30-min treatment with 100 nM bovine insulin (P less than 0.01). 4) Insulin treatment of the cells caused activation of the enzyme glycogen synthase from a glucose-6-phosphate-dependent form to an independent form. Half-maximum activation occurred with 2 nM insulin. These are similar to insulin-induced effects in some mammalian cells. In contrast, no insulin-induced effect on glucose transport could be demonstrated in these cells.
向无细胞壁的粗糙脉孢菌（“黏液菌”）变种FGSC4761的细胞中添加牛胰岛素，对糖原代谢产生了几个显著影响。1）添加胰岛素后30分钟内，糖原前体UDP-葡萄糖的细胞内水平下降了17 - 18%（P小于0.01）。2）用胰岛素培养的细胞比对照细胞的糖原含量多40%。3）用100 nM牛胰岛素处理30分钟后，14C标记的葡萄糖掺入糖原的量增加了41%（P小于0.01）。4）对细胞进行胰岛素处理导致糖原合酶从依赖葡萄糖-6-磷酸的形式激活为独立形式。2 nM胰岛素时出现半数最大激活。这些与某些哺乳动物细胞中胰岛素诱导的效应相似。相比之下，在这些细胞中未证明胰岛素对葡萄糖转运有诱导作用。
