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利用偏振和去偏振动态光散射研究水溶液中的棒状胆固醇胶束。

Rod-like cholesterol micelles in aqueous solution studied using polarized and depolarized dynamic light scattering.

作者信息

Castanho M A, Brown W, Prieto M J

机构信息

Centro de Quimica Fisica Molecular, Lisboa, Portugal.

出版信息

Biophys J. 1992 Dec;63(6):1455-61. doi: 10.1016/S0006-3495(92)81733-2.

Abstract

Micelles of cholesterol in aqueous solution have been investigated using polarized and depolarized dynamic light scattering. They are shown to be highly extended and characterized by a narrow size distribution. It is shown that a rod-like model is applicable with length, L = 580 nm. Determination of the rotational diffusion coefficient by analysis of the autocorrelation function gave a value of theta = 150 s-1, which is close to the calculated value for the rod with this dimension. Depolarized dynamic light scattering measurements as a function of angle gave a value of 110 s-1.

摘要

已使用偏振和去偏振动态光散射研究了胆固醇在水溶液中的胶束。结果表明,它们高度伸展且尺寸分布狭窄。研究表明,棒状模型适用,长度L = 580 nm。通过自相关函数分析确定的旋转扩散系数值为θ = 150 s-1,这与该尺寸棒的计算值相近。作为角度函数的去偏振动态光散射测量给出的值为110 s-1。

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本文引用的文献

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Cholesterol behavior in human serum lipoproteins.
Biochemistry. 1982 Mar 16;21(6):1249-54. doi: 10.1021/bi00535a022.
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The solubility of cholesterol and its exchange between membranes.胆固醇的溶解性及其在膜之间的交换。
Biochim Biophys Acta. 1984 Jan 11;769(1):187-96. doi: 10.1016/0005-2736(84)90022-1.
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Self-association of cholesterol in aqueous solution.胆固醇在水溶液中的自缔合。
Proc Natl Acad Sci U S A. 1973 Aug;70(8):2313-6. doi: 10.1073/pnas.70.8.2313.
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Cholesterol and the cell membrane.胆固醇与细胞膜。
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