Muhonen T
Department of Radiotherapy and Oncology, Helsinki University Central Hospital, Finland.
Melanoma Res. 1992 Nov;2(4):247-52. doi: 10.1097/00008390-199211000-00005.
The prognostic value of cellular DNA content of melanoma metastases was investigated in tumours from 114 consecutive patients referred to the Helsinki University Central Hospital Melanoma Team. Thirty-six percent of the tumours were diploid and 64% aneuploid. For 91 patients the S-phase fraction was calculable. Tumour ploidy and S-phase fraction (SPF) were shown by multivariate Cox model analysis to be independent prognostic variables and major determinants of survival after first recurrence. Patients with either aneuploid or low SPF tumours survived longer than did those with diploid or high SPF tumours. By combining DNA ploidy and SPF, three types of DNA histograms could be defined, associated with favourable, intermediate and poor prognosis. Patients with aneuploid, low SPF metastases showed a median survival of 57 months, whereas the high-risk group with diploid, high SPF metastases survived only 13 months. When ploidy, SPF, age, sex, TNM stage and duration of disease-free survival were analysed as covariates the division of flow cytometry histograms into these three types resulted in the most significant prognostic factor (p < 0.001) in the Cox multivariate analysis.
对赫尔辛基大学中心医院黑色素瘤治疗团队接诊的114例连续患者的肿瘤进行研究，以探讨黑色素瘤转移灶细胞DNA含量的预后价值。36%的肿瘤为二倍体，64%为非整倍体。91例患者的S期细胞分数可计算。多变量Cox模型分析显示，肿瘤倍体和S期细胞分数（SPF）是独立的预后变量，也是首次复发后生存的主要决定因素。非整倍体或低SPF肿瘤患者的生存期比二倍体或高SPF肿瘤患者更长。通过结合DNA倍体和SPF，可定义三种类型的DNA直方图，分别与预后良好、中等和不良相关。非整倍体、低SPF转移灶患者的中位生存期为57个月，而二倍体、高SPF转移灶的高危组患者仅存活13个月。当将倍体、SPF、年龄、性别、TNM分期和无病生存期作为协变量进行分析时，在Cox多变量分析中，将流式细胞术直方图分为这三种类型是最显著的预后因素（p<0.001）。
