消化性溃疡中循环免疫复合物的检测与特征分析。

Detection and characterization of circulating immune complexes in peptic ulcer.

作者信息

Begum S, Ishaq M, Habibullah C M

机构信息

Department of Genetics, Osmania University, Hyderabad, Andhra Pradesh, India.

出版信息

Natl Med J India. 1992 Nov-Dec;5(6):262-5.

PMID:1490153

Abstract

BACKGROUND

Immunological factors have been implicated in the pathogenesis of peptic ulcer following the demonstration of autoantibodies against IgA in patients with this disease. We investigated whether circulating immune complexes were present in this condition, and what type of immunoglobulins were involved.

METHODS

The sera of 37 patients with duodenal ulcer and 8 with gastric ulcer were tested for the presence of circulating immune complexes using the polyethylene glycol (PEG) assay and the results compared with those in 79 controls. The precipitate was dissociated and the levels of IgA, IgG and IgM estimated using the single radial immunodiffusion technique. Simultaneous estimation of these immunoglobulins in the serum was also done. Autoantibodies against IgA in the serum were tested using the ELISA test.

RESULTS

Eleven patients (6 with duodenal ulcer and 5 with gastric ulcer) were found to have circulating immune complexes. The mean protein content of the PEG precipitates was significantly higher in patients than in control subjects (p < 0.001). The mean values of all the immunoglobulin isotypes were higher in patients than in controls. The IgA content in the PEG precipitates of positive cases was higher than that in control subjects (p < 0.05).

CONCLUSION

Patients with peptic ulcer have circulating immune complexes which may interfere with normal immunoregulation.

摘要

背景

在患有消化性溃疡的患者中发现抗IgA自身抗体后，免疫因素被认为与消化性溃疡的发病机制有关。我们研究了这种情况下是否存在循环免疫复合物，以及涉及何种类型的免疫球蛋白。

方法

使用聚乙二醇（PEG）分析法检测37例十二指肠溃疡患者和8例胃溃疡患者血清中循环免疫复合物的存在情况，并将结果与79例对照者的结果进行比较。解离沉淀物，并使用单向放射免疫扩散技术估计IgA、IgG和IgM的水平。同时也对血清中的这些免疫球蛋白进行了估计。使用ELISA试验检测血清中抗IgA自身抗体。