Adelman M R
Department of Anatomy and Cell Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799.
Cell Biol Int Rep. 1992 Nov;16(11):1055-60. doi: 10.1016/s0309-1651(05)80029-3.
The Amoeboflagellate Transformation (AFT) of Physarum polycephalum involves rapid changes in the cytoskeleton, cell shape and cell motility. Use of pharmacologic agents to probe the role of cytoskeletal elements in the AFT are impeded because the transforming cells are very sensitive to such commonly-used drug solvents as DMSO. The anti-microtubule agent tubulozole is found to disrupt, rapidly and transiently, the AFT, inhibiting flagella formation, cell elongation and the arrangement of microtubules and microfilaments. Cells recover quickly, possibly due to precipitation of the drug; the reappearance of normal arrays of microfilaments and cytoplasmic microtubules lags behind flagella formation.
多头绒泡菌的变形虫鞭毛虫转化（AFT）涉及细胞骨架、细胞形状和细胞运动性的快速变化。由于转化细胞对二甲基亚砜等常用药物溶剂非常敏感，因此使用药理试剂来探究细胞骨架成分在AFT中的作用受到阻碍。发现抗微管药物tubulozole能快速且短暂地破坏AFT，抑制鞭毛形成、细胞伸长以及微管和微丝的排列。细胞恢复迅速，可能是由于药物沉淀；微丝和细胞质微管正常排列的重新出现落后于鞭毛形成。
