Stracke M L, Soroush M, Liotta L A, Schiffmann E
National Institutes of Health, National Cancer Institute, Laboratory of Pathology, Bethesda, Maryland.
Kidney Int. 1993 Jan;43(1):151-7. doi: 10.1038/ki.1993.25.
Cytoskeletal agents have been demonstrated to inhibit stimulated motility and substrate adherence by the human tumor cell line, A2058. cis-tubulozole, taxol, and cytochalasin D were tested for their effects on chemotaxis in response to a tumor cytokine, autocrine motility factor, and on adherence to several substrata: laminin- and gelatin-coated dishes as well as tissue culture plastic. Cytochalasin D, which inhibits microfilament polymerization, abolished stimulated motility. Taxol, which stabilizes microtubules, decreased stimulated motility to a greater degree than cis-tubulozole, which inhibits microtubular polymerization. In contrast, cis-tubulozole had the greatest inhibitory effect on adherence with a gelatin substratum more affected (100% inhibition) than tissue culture plastic (90%) or laminin substratum (52%). Taxol affected adherence in the same order but less than cis-tubulozole. Cytochalasin D had no significant effect on adherence to laminin with moderate inhibition of adherence to tissue culture plastic or gelatin. These data suggest that, in these tumor cells, microfilaments are more crucial for motility than adherence, but the dynamic polymerization and depolymerization of microtubules are required for both types of cellular activities.
细胞骨架药物已被证明可抑制人肿瘤细胞系A2058的刺激运动性和底物黏附。测试了顺式微管唑、紫杉醇和细胞松弛素D对趋化性的影响，该趋化性是对肿瘤细胞因子、自分泌运动因子的反应，还测试了它们对几种底物的黏附作用：层粘连蛋白和明胶包被的培养皿以及组织培养塑料。抑制微丝聚合的细胞松弛素D消除了刺激运动性。稳定微管的紫杉醇比抑制微管聚合的顺式微管唑更能降低刺激运动性。相比之下，顺式微管唑对黏附的抑制作用最大，对明胶底物的影响更大（100%抑制），比对组织培养塑料（90%）或层粘连蛋白底物（52%）的影响更大。紫杉醇对黏附的影响顺序相同，但小于顺式微管唑。细胞松弛素D对层粘连蛋白黏附无显著影响，对组织培养塑料或明胶黏附有中度抑制作用。这些数据表明，在这些肿瘤细胞中，微丝对运动性比对黏附更关键，但微管的动态聚合和解聚对于这两种细胞活动都是必需的。
