Sarati S, Caccia S
Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
Eur J Drug Metab Pharmacokinet. 1992 Jul-Sep;17(3):205-11. doi: 10.1007/BF03190147.
A new high performance liquid chromatographic procedure has been developed for the simultaneous quantification of piribedil (PD) and its three main basic metabolites in rat plasma and urine, without and after hydrolysis. The procedure relies on isolation of the compounds from plasma and urine constituents using the Sep-Pak C18 cartridge, with satisfactory recovery and specificity, and resolution by acetonitrile gradient elution on a C18 reversed phase column coupled to a UV detector monitored at 240 nm. The assay was linear over a wide range of concentrations for all compounds in both body fluids with mean within-day and day-to-day coefficient of variation (CV) and relative error (RE) generally below 10%. Plasma concentrations of PD and its metabolites at selected intervals and urinary recoveries of all compounds before and after enzymatic hydrolysis are presented.
已开发出一种新的高效液相色谱法，用于同时定量大鼠血浆和尿液中未经水解和水解后的匹立哌唑（PD）及其三种主要碱性代谢物。该方法依靠使用Sep-Pak C18柱从血浆和尿液成分中分离化合物，回收率和特异性良好，并通过在连接紫外检测器（监测波长为240 nm）的C18反相柱上进行乙腈梯度洗脱来实现分离。对于两种体液中的所有化合物，该测定法在很宽的浓度范围内呈线性，日内和日间平均变异系数（CV）和相对误差（RE）通常低于10%。文中给出了选定时间间隔时PD及其代谢物的血浆浓度，以及酶水解前后所有化合物的尿回收率。
