Bosler E M, Evans R D, Schneider E M
New York State Department of Health, Health Sciences Center, State University of New York, Stony Brook.
FEMS Microbiol Lett. 1992 Dec 1;78(2-3):317-9. doi: 10.1016/0378-1097(92)90047-r.
A punch biopsy method was developed to detect and cultivate Borrelia burgdorferi from canine ear tissue. The biopsy procedure was sensitive in that spirochetes were detected in 100% of the dogs upon which infected ticks had fed. B. burgdorferi could be cultured as early as 6 days after tick feeding at which time the organisms could not be concurrently recovered from peripheral blood. The ear punch biopsy provides a reliable method for sampling live canines and should prove useful for detecting early B. burgdorferi infection and for monitoring duration of non-disseminated infection. Additionally, the procedure will aid in the development of an animal model for evaluation of preventive and therapeutic treatment modalities.
已开发出一种穿孔活检方法，用于从犬耳部组织中检测和培养伯氏疏螺旋体。该活检程序具有敏感性，因为在所有被感染蜱叮咬过的犬只中均检测到了螺旋体。早在蜱叮咬后6天就可以培养出伯氏疏螺旋体，此时在外周血中无法同时检测到这些病原体。耳部穿孔活检为活体犬采样提供了一种可靠的方法，并且应该被证明对检测早期伯氏疏螺旋体感染以及监测非播散性感染的持续时间有用。此外，该程序将有助于开发一种动物模型，用于评估预防和治疗方式。
