WILSON A T, ROSENBLUM H
J Exp Med. 1952 Jan;95(1):39-50. doi: 10.1084/jem.95.1.39.
Lactenin is reversibly inactivated by the exclusion of atmospheric oxygen. It is also inactivated by the sulfur-containing reducing agents cysteine, glutathione, thioglycollic acid, and BAL. Group A streptococci which have been acted upon by lactenin have been killed, and not merely prevented from multiplying, since they cannot be revived by inactivating lactenin through the addition of a reducing agent. Thiamine in great excess inactivates lactenin. The mechanism by which it accomplishes this has not been discovered, but it suggests that the mode of action of lactenin may be to deny thiamine to the lactenin-sensitive cell. Lactenin sensitivity is not, however, related to a requirement for exogenous thiamine, nor does lactenin appear to function by binding environmental thiamine in a form unavailable to the sensitive cell.
乳链菌肽可通过排除大气中的氧气而被可逆性灭活。它也可被含硫还原剂半胱氨酸、谷胱甘肽、巯基乙酸和二巯基丙醇灭活。受乳链菌肽作用的A组链球菌已被杀死,而不仅仅是被阻止繁殖,因为通过添加还原剂使乳链菌肽失活并不能使它们复活。大量过量的硫胺素会使乳链菌肽失活。其实现这一作用的机制尚未被发现,但这表明乳链菌肽的作用方式可能是使对乳链菌肽敏感的细胞无法获得硫胺素。然而,对乳链菌肽的敏感性与对外源硫胺素的需求无关,乳链菌肽似乎也不是通过以敏感细胞无法利用的形式结合环境中的硫胺素来发挥作用的。