牛奶中乳过氧化物酶-硫氰酸盐-过氧化氢系统对大肠杆菌和一些革兰氏阴性病原菌的非特异性杀菌活性。
Nonspecific bactericidal activity of the lactoperoxidases-thiocyanate-hydrogen peroxide system of milk against Escherichia coli and some gram-negative pathogens.
作者信息
Reiter B, Marshall V M, Rosén C G
出版信息
Infect Immun. 1976 Mar;13(3):800-7. doi: 10.1128/iai.13.3.800-807.1976.
Abstract
Two strains of Escherichia coli and one strain each of Salmonella typhimurium and Pseudomonas aeruginosa were killed by the bactericidal activity of the lactoperoxidase-thiocyanate-hydrogen peroxide system in milk and in a synthetic medium. H2O2 was supplied exogenously by glucose oxidase, and glucose was produced at a level which was itself noninhibitory. Two phases were distinguished: the first phase was dependent on the oxidation of SCN(-) by lactoperoxidase and H2O2, which was reversed by reducing agent, and the second phase was dependent on the presence of accumulated H2O2, which was reversed by catalase. The latter enzyme could also reverse the first phase, but only when present in excessive and unphysiological levels. The bactericidal activity was greatest at pH 5 and below, and it depended on the SCN(-)concentration and on the number of organisms. Since raw or heated milk neutralizes the acid barrier against infection in the stomach, the bactericidal system discussed may contribute to the prevention of enteric infections in neonates.
摘要
在牛奶和合成培养基中，乳过氧化物酶 - 硫氰酸盐 - 过氧化氢体系的杀菌活性杀死了两株大肠杆菌以及一株鼠伤寒沙门氏菌和一株铜绿假单胞菌。过氧化氢由葡萄糖氧化酶外源提供，葡萄糖的产生水平本身并无抑制作用。可区分出两个阶段：第一阶段依赖于乳过氧化物酶和过氧化氢对硫氰根离子的氧化，还原剂可使其逆转；第二阶段依赖于积累的过氧化氢的存在，过氧化氢酶可使其逆转。后一种酶也可逆转第一阶段，但只有在过量且非生理水平存在时才会如此。杀菌活性在pH 5及以下时最强，它取决于硫氰根离子浓度和微生物数量。由于生牛奶或加热后的牛奶会中和胃中抵御感染的酸性屏障，所讨论的杀菌系统可能有助于预防新生儿的肠道感染。
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