Teichert J, Preiss R
Institute of Clinical Pharmacology, University of Leipzig, Germany.
Int J Clin Pharmacol Ther Toxicol. 1992 Nov;30(11):511-2.
A method has been developed for the HPLC analysis of lipoic acid and its reduced form (dihydrolipoic acid) in biological samples. Both substances are released from the samples by enzymatic hydrolysis and extracted by solid phase column. The extracts, after evaporation, were chromatographed and quantified by electrochemical detection. The basic level was in the range 1-25 ng/ml for lipoic acid and 33-145 ng/ml for dihydrolipoic acid (6 healthy volunteers).
已开发出一种用于高效液相色谱法分析生物样品中硫辛酸及其还原形式（二氢硫辛酸）的方法。这两种物质通过酶促水解从样品中释放出来，并通过固相柱进行萃取。萃取物经蒸发后进行色谱分析，并通过电化学检测进行定量。硫辛酸的基础水平在1 - 25 ng/ml范围内，二氢硫辛酸的基础水平在33 - 145 ng/ml范围内（6名健康志愿者）。
