Puente M E, Vega-Villasante F, Holguin G, Bashan Y
Department of Microbiology, Center for Biological Research, La Paz, Mexico.
J Appl Bacteriol. 1992 Dec;73(6):465-71. doi: 10.1111/j.1365-2672.1992.tb05006.x.
Adults and nauplii of the brine shrimp, Artemia, together with Vibrio parahaemolyticus, were placed in sewage-contaminated sea-water which had been treated with chlorine dioxide (Hallox E-100TM) to test its potential as a disinfectant for salt water aquaculture. The nauplii were very susceptible to low concentrations of chlorine dioxide (47 micrograms/l Cl-), but the adults were slightly more resistant. Sterile sea-water treated with lower concentrations of chlorine dioxide (less than 47 micrograms/l Cl-) had no effect on the shrimp, but inhibited the growth of V. parahaemolyticus. In sewage-contaminated sea-water, chlorine dioxide levels of 285-2850 micrograms/l, necessary for the inactivation of V. parahaemolyticus and any native bacteria, destroyed the Artemia culture. Hallox E-100TM persisted in sea-water for 18 h, but later decayed. We conclude that: (i) Artemia nauplii are a sensitive and convenient test-organism to determine low concentrations of chlorine dioxide in sea-water; (ii) chlorine dioxide is efficient for controlling V. parahaemolyticus in sea-water; and (iii) chlorine dioxide should be further evaluated as a potential disinfectant for aquaculture, but, for higher organisms than Artemia.
将卤虫（丰年虫）的成体和无节幼体与副溶血性弧菌一起置于经二氧化氯（Hallox E - 100TM）处理过的受污水污染的海水中，以测试其作为咸水养殖消毒剂的潜力。无节幼体对低浓度的二氧化氯（47微克/升Cl-）非常敏感，但成体的耐受性稍强。用较低浓度的二氧化氯（低于47微克/升Cl-）处理的无菌海水对虾没有影响，但抑制了副溶血性弧菌的生长。在受污水污染的海水中，使副溶血性弧菌和任何天然细菌失活所需的二氧化氯水平为285 - 2850微克/升，这会破坏卤虫培养物。Hallox E - 100TM在海水中持续存在18小时，但随后会降解。我们得出以下结论：（i）卤虫无节幼体是一种灵敏且方便的测试生物，可用于测定海水中低浓度的二氧化氯；（ii）二氧化氯对控制海水中的副溶血性弧菌有效；（iii）应进一步评估二氧化氯作为水产养殖潜在消毒剂的可能性，但针对的是比卤虫更高等的生物。
