Harakeh M S, Berg J D, Hoff J C, Matin A
Appl Environ Microbiol. 1985 Jan;49(1):69-72. doi: 10.1128/aem.49.1.69-72.1985.
The resistance of bacteria to antimicrobial agents could be influenced by growth environment. The susceptibility of two enteric bacteria, Yersinia enterocolitica and Klebsiella pneumoniae, to chlorine dioxide was investigated. These organisms were grown in a defined medium in a chemostat and the influence of growth rate, temperature, and cell density on the susceptibility was studied. All inactivation experiments were conducted with a dose of 0.25 mg of chlorine dioxide per liter in phosphate-buffered saline at pH 7.0 and 23 degrees C. The results indicated that populations grown under conditions that more closely approximate natural aquatic environments, e.g., low temperatures and growth at submaximal rates caused by nutrient limitation, were most resistant. The conclusion from this study is that antecedent growth conditions have a profound effect on the susceptibility of bacteria to disinfectants, and it is more appropriate to use the chemostat-grown bacteria as test organisms to evaluate the efficacy of a certain disinfectant.
细菌对抗菌剂的耐药性可能受生长环境影响。研究了两种肠道细菌——小肠结肠炎耶尔森菌和肺炎克雷伯菌对二氧化氯的敏感性。这些微生物在恒化器中的特定培养基中培养，并研究了生长速率、温度和细胞密度对敏感性的影响。所有灭活实验均在pH 7.0、23℃的磷酸盐缓冲盐水中，以每升0.25毫克二氧化氯的剂量进行。结果表明，在更接近自然水生环境的条件下生长的菌群，例如低温以及营养限制导致的亚最大生长速率下生长的菌群，耐药性最强。该研究的结论是，先前的生长条件对细菌对消毒剂的敏感性有深远影响，使用恒化器培养的细菌作为测试生物来评估某种消毒剂的效果更为合适。
