Manju Sivalingam, Malaikozhundan Balasubramanian, Withyachumnarnkul Boonsirm, Vaseeharan Baskaralingam
Biomaterials and Biotechnology in Animal Health Lab, Crustacean Molecular Biology and Genomics Division, Department of Animal Health and Management, Alagappa University, Karaikudi 630 004, Tamil Nadu, India.
Department of Anatomy and Centex Shrimp, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; Aquatic Animal Biotechnology Research Center, Faculty of Science and Industrial Technology, Prince of Songkla University, Surat Thani Campus, Surat Thani 84100, Thailand.
J Invertebr Pathol. 2016 May;136:43-9. doi: 10.1016/j.jip.2016.03.004. Epub 2016 Mar 2.
The anti-Vibrio activity of essential oils (EOs) of nine medicinal plants was tested against 28 Vibrio spp. isolated from diseased Fenneropenaeus indicus. EO of Nigella sativa exhibited anti-Vibrio activity against all Vibrio spp. and greater inhibition was noted for the isolate V2 which was identified as Vibrio parahaemolyticus Dahv2. Further, EO of N. sativa effectively inhibited V. parahaemolyticus Dahv2 with an inhibition zone of 23.9mm at 101.2μgml(-1). Moreover, EO of N. sativa revealed anti-biofilm activity at 101.2μgml(-1) against V. parahaemolyticus Dahv2 and inhibited the growth of V. parahaemolyticus Dahv2 at 100μgml(-1).In vivo experimental infection studies showed that the survival of Artemia spp. infected with V. parahaemolyticus Dahv2 at 1×10(3)cfuml(-1) was only 40%. However, the survival of Artemia spp. was significantly increased after challenge with 100μgml(-1) of EO of N. sativa. EO of N. sativa showed higher anti-oxidant potential and total phenol content than other EOs tested. The anti-oxidant activity of EO of N. sativa was highly correlated to their total phenolic contents (r=0.836, P<0.05). This observation suggests that EO of N. sativa protected the Artemia spp. after experimental infection of V. parahaemolyticus Dahv2.
测试了9种药用植物精油（EOs）对从患病印度对虾中分离出的28种弧菌属细菌的抗弧菌活性。黑种草精油对所有弧菌属细菌均表现出抗弧菌活性，对鉴定为副溶血性弧菌Dahv2的分离株V2的抑制作用更强。此外，黑种草精油在101.2μg/ml时能有效抑制副溶血性弧菌Dahv2，抑菌圈为23.9mm。此外，黑种草精油在101.2μg/ml时对副溶血性弧菌Dahv2具有抗生物膜活性，并在100μg/ml时抑制副溶血性弧菌Dahv2的生长。体内实验感染研究表明，感染1×10³cfu/ml副溶血性弧菌Dahv2的卤虫属动物的存活率仅为40%。然而，用100μg/ml黑种草精油攻击后，卤虫属动物的存活率显著提高。黑种草精油比其他测试的精油表现出更高的抗氧化潜力和总酚含量。黑种草精油的抗氧化活性与其总酚含量高度相关（r=0.836，P<0.05）。这一观察结果表明，黑种草精油在副溶血性弧菌Dahv2实验感染后保护了卤虫属动物。
