Felscher D, Wulfmeyer M
Institut für Rechtsmedizin des Universitätsklinikums Carl Gustav Carus der Technischen Universität Dresden, Germany.
J Anal Toxicol. 1998 Sep;22(5):363-6. doi: 10.1093/jat/22.5.363.
This study shows a simple, rapid, and specific method for the quantitative determination of cyanide ion in body fluids, especially blood, by fluorimetry. It is based upon the transformation of cyanide ion into hydrocyanic acid, which then reacts with 2,3-naphthalenedialdehyde and taurine in a self-contained system. The 1-cyano-2-benzoisoindole derivate thus formed is suitable for fluorimetric measurement (lambdaEX = 418 nm; lambdaEM = 460 nm). The fluorescence intensity can be determined by spectrophotometry or by high-performance liquid chromatography (HPLC) with fluorescence detection. The detection limit is 0.002 microg/mL. Linearity was excellent from 0.002 to 1 microg/mL for spectrophotometry and from 0.002 to 5 microg/mL for HPLC with fluorescence detection. The coefficient of variation for repeatability was 8% or less. Thiocyanate and sulfide did not interfere, even at high concentrations (200 microg/mL). The method was applicable to whole blood, so it should be suitable for both clinical and forensic purposes.
本研究展示了一种通过荧光法对体液尤其是血液中的氰离子进行定量测定的简单、快速且特异的方法。它基于氰离子转化为氢氰酸，然后氢氰酸在一个自成体系中与2,3-萘二醛和牛磺酸发生反应。由此形成的1-氰基-2-苯并异吲哚衍生物适用于荧光测定（激发波长λEX = 418 nm；发射波长λEM = 460 nm）。荧光强度可通过分光光度法或带荧光检测的高效液相色谱法（HPLC）来测定。检测限为0.002微克/毫升。分光光度法在0.002至1微克/毫升范围内线性良好，带荧光检测的HPLC在0.002至5微克/毫升范围内线性良好。重复性的变异系数为8%或更低。硫氰酸盐和硫化物即使在高浓度（200微克/毫升）下也不产生干扰。该方法适用于全血，因此应适用于临床和法医目的。
