Alliegro M A, Dyer K D, Cragoe E J, Glaser B M, Alliegro M C
Retina Center, St. Joseph Hospital, Baltimore, MD 21284.
J Chromatogr. 1992 Nov 6;582(1-2):217-23. doi: 10.1016/0378-4347(92)80322-h.
An assay for amiloride was devised for efficient use with the wide variety of analogues available. Amiloride was extracted from 1-ml plasma samples by elution from a C8 preparative column with 6% acetonitrile-45% methanol-5.4% acetic acid, adjusted to pH 4.0 with trimethylamine. Samples were lyophilized, resuspended in 50% methanol, filtered through 0.22-microns Spin-X cartridges, applied to a reversed-phase C18 column, and eluted in a 0-50% acetonitrile gradient in 0.4% acetic acid, pH 4.5 (1.2 ml/min). Detection by ultraviolet absorbance at 360 nm was linear from 1 to 1000 ng. Versatility of the method was demonstrated with the analogues benzamil, 6-hydro-, 6-iodo-, 5-hexamethylene-, and 5-chlorobenzyl-2',4'-dimethylbenzyl-amiloride.
设计了一种用于氨氯地平的分析方法，以便能有效地用于各种可用的类似物。氨氯地平通过用6%乙腈-45%甲醇-5.4%乙酸（用三甲胺调节至pH 4.0）从C8制备柱上洗脱，从1毫升血浆样品中提取。样品冻干后，重悬于50%甲醇中，通过0.22微米的Spin-X滤器过滤，应用于反相C18柱，并在0.4%乙酸（pH 4.5）中以0-50%乙腈梯度洗脱（1.2毫升/分钟）。在360纳米处通过紫外吸光度检测，线性范围为1至1000纳克。用类似物苄甲氯胺、6-羟基-、6-碘-、5-六亚甲基-和5-氯苄基-2',4'-二甲基苄基-氨氯地平证明了该方法的通用性。
