Yip M S, Coates P E, Thiessen J J
J Chromatogr. 1984 May 11;307(2):343-50. doi: 10.1016/s0378-4347(00)84105-5.
A high-performance liquid chromatographic method has been developed for amiloride in rabbit plasma and urine which uses a reversed-phase C18 column, a mobile phase (flow-rate 2 ml/min) consisting of 32% acetonitrile in 0.15 M perchloric acid, pH 2.2, and spectrofluorometric detection via excitation at 286 nm. A simple extraction step with ethyl acetate eliminates interfering peaks. Short retention times of about 2.3 and 3.8 min are observed for amiloride and the internal standard, triamterene, respectively. The method can measure 4 ng/ml amiloride in plasma. This assay has been used to explore the pharmacokinetics of amiloride in rabbits. The plasma disposition profile is biexponential after a 50-mg intravenous bolus dose and there is no evidence for saturable elimination at zero-order infusion rates of 1.8, 3.6 and 7.2 mg/h.
已开发出一种用于测定兔血浆和尿液中阿米洛利的高效液相色谱法，该方法使用反相C18柱，流动相（流速2 ml/分钟）由0.15 M高氯酸中32%的乙腈组成，pH值为2.2，并通过在286 nm处激发进行荧光光谱检测。用乙酸乙酯进行简单萃取步骤可消除干扰峰。阿米洛利和内标氨苯蝶啶的保留时间分别约为2.3分钟和3.8分钟。该方法可测定血浆中4 ng/ml的阿米洛利。此分析方法已用于研究阿米洛利在兔体内的药代动力学。静脉注射50 mg大剂量后，血浆处置曲线呈双指数形式，并且没有证据表明在1.8、3.6和7.2 mg/h的零级输注速率下存在饱和消除。
