Brown L W, Hundt H K, Swart K J
Department of Pharmacology, Faculty of Medicine, UOFS, Bloemfontein, South Africa.
J Chromatogr. 1992 Nov 6;582(1-2):268-72. doi: 10.1016/0378-4347(92)80332-k.
An automated high-performance liquid chromatographic method for the determination of mianserin in plasma is described. Extraction and injection of the samples were automatically done by the Gilson ASPEC system using C8, 100-mg Supelclean solid-phase extraction columns. The extracts were chromatographed on a reversed-phase C18 column (150 mm x 3.9 mm I.D.) with a phosphate buffer-acetonitrile-methanol mobile phase and the analytes detected electrochemically. Calibration curves were linear to at least 53.7 ng/ml at which the between-day relative standard deviation was 5% and the recovery 101%. The limit of quantification was 1.67 ng/ml at which the between-day relative standard deviation was 9% and the recovery 92% using a sample volume of 0.5 ml. The method was applied to the determination of mianserin in the plasma of normal human volunteers participating in a comparative bioavailability study.
本文描述了一种用于测定血浆中米安色林的自动化高效液相色谱法。样品的萃取和进样由吉尔森ASPEC系统自动完成，使用C8、100mg Supelclean固相萃取柱。萃取物在反相C18柱（150mm×3.9mm内径）上进行色谱分析，流动相为磷酸盐缓冲液 - 乙腈 - 甲醇，分析物采用电化学检测。校准曲线在至少53.7ng/ml范围内呈线性，日间相对标准偏差为5%，回收率为101%。使用0.5ml样品体积时，定量限为1.67ng/ml，日间相对标准偏差为9%，回收率为92%。该方法应用于参与比较生物利用度研究的正常人类志愿者血浆中米安色林的测定。
