Swart K J, Papgis M
Department of Pharmacology, Faculty of Medicine, UOFS, Bloemfontein, South Africa.
J Chromatogr. 1992 Feb 28;593(1-2):21-4. doi: 10.1016/0021-9673(92)80260-2.
Due to the unstable nature of rifampicin, a rapid automated high-performance liquid chromatographic method had to be developed for the analysis of a large number of plasma samples generated during a bioavailability trial. Extraction and injection of the samples were automatically done by a sample preparation system using C2, 100 mg Bond Elut extraction columns. The extracts were chromatographed on a 4-microns reversed-phase C18 column with a citrate buffer and acetonitrile as mobile phase. The analytes were detected at 342 nm. Calibration curves were linear to at least 20 micrograms/ml and the limit of quantification was 0.16 micrograms/ml.
由于利福平性质不稳定，必须开发一种快速自动化高效液相色谱法，用于分析生物利用度试验期间产生的大量血浆样本。样品的提取和进样由使用100mg C2 Bond Elut萃取柱的样品制备系统自动完成。提取物在以柠檬酸盐缓冲液和乙腈为流动相的4微米反相C18柱上进行色谱分析。在342nm处检测分析物。校准曲线至少在20微克/毫升范围内呈线性，定量限为0.16微克/毫升。
