固相萃取和高效液相色谱后采用气相色谱法测定尿睾酮

Urinary testosterone measurement by gas chromatography after solid-phase extraction and high-performance liquid chromatography.

作者信息

Venturelli E, Manzari A, Cavalleri A, Benzo M, Secreto G, Marubini E

机构信息

National Cancer Institute, Endocrine Unit, Milan, Italy.

出版信息

J Chromatogr. 1992 Nov 6;582(1-2):7-12. doi: 10.1016/0378-4347(92)80295-2.

DOI:10.1016/0378-4347(92)80295-2

PMID:1491060

Abstract

A method was developed for the rapid determination of testosterone in urine. The procedure consists of solid-phase extraction (SPE) followed by high-performance liquid chromatographic (HPLC) clean-up before gas chromatographic determination. Recovery was evaluated by adding [3H]testosterone (10(4) cpm) to urine samples; the mean recovery of radioactivity after SPE and HPLC was 82%. Precision was estimated by repeated measurement of testosterone in four different urine samples; the coefficient of variation was 7.9% (95% confidence limits 6.1-11.4%). Accuracy was evaluated by standard addition and dilution assays; a linear relationship was found between the expected and observed values (r2 = 0.982). The method is rapid, effective and suitable for routine analysis.

摘要

开发了一种快速测定尿液中睾酮的方法。该程序包括固相萃取（SPE），然后在气相色谱测定之前进行高效液相色谱（HPLC）净化。通过向尿液样本中添加[3H]睾酮（10(4) cpm）来评估回收率；SPE和HPLC后放射性的平均回收率为82%。通过对四个不同尿液样本中的睾酮进行重复测量来估计精密度；变异系数为7.9%（95%置信限6.1 - 11.4%）。通过标准加入和稀释试验评估准确性；预期值和观察值之间发现线性关系（r2 = 0.982）。该方法快速、有效，适用于常规分析。