Omoto C K
Department of Genetics and Cell Biology, Washington State University, Pullman 99164-4234.
J Muscle Res Cell Motil. 1992 Dec;13(6):635-9. doi: 10.1007/BF01738253.
The axonemal motion supported by fluorescent ribose-modified analogues, anthraniloyl ATP (Ant-ATP) and methylanthraniloyl ATP (Mant-ATP), was investigated. Ant-ATP and Mant-ATP supported good vigorous motion. A detailed study of the movement shows that the maximum beat frequencies (Vmax) were significantly lower with the analogues. However, Michaelis constants (Km) for beat frequency were also significantly lower than with ATP. Thus the net effect of changes in these two parameters, Vmax/Km, was similar for ATP and Ant-ATP and higher with Mant-ATP. Thus these fluorescent analogues are good substrates for axonemal movement. The consistently higher value of Vmax/Km, a measure of substrate selectivity, with Mant-ATP over Ant-ATP suggests a feature of the ribose binding site. Other significant differences in the movement with the fluorescent analogues are quantified in terms of kinetic measures of sliding velocity and bend propagation velocity.
研究了由荧光核糖修饰类似物邻氨基苯甲酰基ATP（Ant-ATP）和甲基邻氨基苯甲酰基ATP（Mant-ATP）支持的轴丝运动。Ant-ATP和Mant-ATP支持良好的剧烈运动。对运动的详细研究表明，类似物的最大搏动频率（Vmax）明显较低。然而，搏动频率的米氏常数（Km）也明显低于ATP。因此，这两个参数Vmax/Km变化的净效应，对于ATP和Ant-ATP是相似的，而对于Mant-ATP则更高。因此，这些荧光类似物是轴丝运动的良好底物。Mant-ATP相对于Ant-ATP的Vmax/Km（底物选择性的一种度量）持续较高的值表明了核糖结合位点的一个特征。用荧光类似物进行运动时的其他显著差异，根据滑动速度和弯曲传播速度的动力学测量进行了量化。
