Inglis A S, Duncan M W, Adams P, Tseng A
Garvan Institute of Medical Research, St. Vincent's Hospital, Darlinghurst, NSW, Australia.
J Biochem Biophys Methods. 1992 Oct;25(2-3):163-71. doi: 10.1016/0165-022x(92)90008-x.
Pure amino acid thiohydantoins are required as reference standards for development of C-terminal-sequencing procedures based on thiohydantoin formation of the C-terminal amino acids of peptides and proteins. Proline thiohydantoin was prepared using a straightforward method involving reaction of acetylproline with ammonium thiocyanate. It was characterized by UV spectrophotometry, mass spectrometry and back-hydrolysis to the free amino acid. These data establish unequivocally that the thiocyanate procedure is applicable to proline as well as to the other common amino acids. This work also validates earlier claims that proline thiohydantoin can be prepared by reaction with thiocyanic acid.
纯氨基酸乙内酰硫脲是基于肽和蛋白质C端氨基酸形成乙内酰硫脲来开发C端测序程序所需的参考标准。脯氨酸乙内酰硫脲是通过一种直接的方法制备的，该方法涉及乙酰脯氨酸与硫氰酸铵的反应。通过紫外分光光度法、质谱法以及水解回游离氨基酸对其进行了表征。这些数据明确证实硫氰酸盐程序适用于脯氨酸以及其他常见氨基酸。这项工作还验证了早期关于脯氨酸乙内酰硫脲可通过与硫氰酸反应制备的说法。
