Mo B, Li J, Liang S
Department of Biology, Hunan Normal University, Changsha, People's Republic of China.
Anal Biochem. 1997 Jul 1;249(2):207-11. doi: 10.1006/abio.1997.2156.
A novel and efficient method to prepare amino acid thiohydantoins, which are required as reference standards for development of C-terminal protein sequencing, is reported. Amino acid thiohydantoins were prepared using a straightforward method involving reaction of 20 free amino acids with acetyl chloride as activating reagent and trimethylsilyl isothiocyanate (TMS-ITC) as derivatizing reagent. The products were characterized by HPLC, uv spectra, amino acid analysis, MS, and NMR. Different reaction conditions were investigated and the chemical mechanism of the formation of amino acid thiohydantoins was illustrated.
报道了一种制备氨基酸硫代乙内酰脲的新颖高效方法，氨基酸硫代乙内酰脲是用于C端蛋白质测序开发的参考标准品所必需的。氨基酸硫代乙内酰脲通过一种直接的方法制备，该方法涉及20种游离氨基酸与作为活化试剂的乙酰氯以及作为衍生化试剂的三甲基硅基异硫氰酸酯（TMS-ITC）反应。产物通过高效液相色谱、紫外光谱、氨基酸分析、质谱和核磁共振进行表征。研究了不同的反应条件，并阐明了氨基酸硫代乙内酰脲形成的化学机理。
