Beever R E, Burns D J
J Bacteriol. 1978 Jun;134(3):1176-8. doi: 10.1128/jb.134.3.1176-1178.1978.
Addition of cycloheximide to Neurospora crassa germlings growing in liquid medium caused an exponential loss of phosphate uptake activity (half-life, ca. 2 h). No loss of activity resulted when germlings were resuspended, at the time of cycloheximide addition, in medium of a substantially lower phosphate concentration. It is concluded that the phosphate uptake systems are not subject to rapid turnover.
向生长在液体培养基中的粗糙脉孢菌芽孢添加放线菌酮会导致磷酸盐摄取活性呈指数下降(半衰期约为2小时)。当在添加放线菌酮时将芽孢重悬于磷酸盐浓度低得多的培养基中时,活性没有损失。由此得出结论,磷酸盐摄取系统不会快速更新。