Mu噬菌体诱导的大肠杆菌unc操纵子中的极性现象
Mu-induced polarity in the unc operon of Escherichia coli.
作者信息
Gibson F, Downie J A, Cox G B, Radik J
出版信息
J Bacteriol. 1978 Jun;134(3):728-36. doi: 10.1128/jb.134.3.728-736.1978.
Abstract
Mutant strains of Escherichia coli were isolated in which mutator (Mu) phage was inserted into various unc genes. Partial diploid strains were prepared from each of the Mu-induced unc mutants by using F-plasmids carrying mutations in one of the known unc genes (uncA, uncB, uncC, or uncD). The partial diploid strains and the corresponding segregant strains were examined for their ability to grow on succinate. The aerobic growth yields on limiting concentrations of glucose were also determined. Magnesium-stimulated adenosine triphosphatase activities, ATP-dependent transhydrogenase activities, and Atebrin fluorescence quenching activities were determined by using membrane preparations from each strain. Genetic complementation was assessed from the results obtained, and it was concluded that the four unc genes examined are part of a single transcriptional unit and that they are transcribed in the order uncBADC.
摘要
分离出了大肠杆菌的突变菌株，其中诱变（Mu）噬菌体插入到了各种unc基因中。通过使用携带已知unc基因（uncA、uncB、uncC或uncD）之一突变的F质粒，从每个Mu诱导的unc突变体中制备了部分二倍体菌株。检测了部分二倍体菌株和相应的分离菌株在琥珀酸盐上生长的能力。还测定了在有限浓度葡萄糖上的有氧生长产量。通过使用每个菌株的膜制剂，测定了镁刺激的三磷酸腺苷酶活性、ATP依赖性转氢酶活性和阿的平荧光猝灭活性。根据所得结果评估了遗传互补性，得出结论：所检测的四个unc基因是单个转录单元的一部分，并且它们按uncBADC的顺序转录。
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