Gibson F, Cox G B, Downie J A, Radik J
Biochem J. 1977 Mar 15;162(3):665-70. doi: 10.1042/bj1620665.
A plasmid was isolated which included the region of the Escherichia coli chromosome carrying the known genes concerned with oxidative phosphorylation (unc genes). This plasmid was used to prepare partial diploids carrying normal unc alleles on the episome and one of the three mutant alleles (unc A401, uncB402 or unc-405) on the chromosome. These strains were compared with segregants from which the plasmid had been lost. Dominance of either normal ormutant unc alleles was determined by growth on succinate, growth yields on glucose, Mg-ATPase (Mg2+-stimulated adenosine triphosphatase) activity, atebrin-fluorescence quenching, ATP-dependent transhydrogenase activity and oxidative phosphorylation. In all the above tests, dominance of the normal allele was observed. However, in membranes from the diploid strains which carried a normal allele and either of the mutant alleles affecting Mg-ATPase activity (uncA401 or unc-405), the energy-linked functions were only partially restored.
分离出了一种质粒，其包含大肠杆菌染色体上携带与氧化磷酸化相关已知基因（unc基因）的区域。该质粒用于制备部分二倍体，这些部分二倍体在附加体上携带正常的unc等位基因，在染色体上携带三个突变等位基因之一（uncA401、uncB402或unc - 405）。将这些菌株与丢失了质粒的分离株进行比较。通过在琥珀酸盐上的生长、在葡萄糖上的生长产量、镁 - ATP酶（Mg2 +刺激的腺苷三磷酸酶）活性、阿的平荧光猝灭、ATP依赖性转氢酶活性和氧化磷酸化来确定正常或突变unc等位基因的显性。在上述所有测试中，均观察到正常等位基因的显性。然而，在携带正常等位基因和影响镁 - ATP酶活性的突变等位基因之一（uncA401或unc - 405）的二倍体菌株的膜中，能量相关功能仅部分恢复。
