Chiba M
Department of Orthopedic Surgery, Akita University School of Medicine, Japan.
Nihon Seikeigeka Gakkai Zasshi. 1992 Dec;66(12):1245-54.
The distribution of adrenergic nerve fibers in the vessels, nerves, and skeletal muscles of the rat was studied by the glyoxylic acid fluorescence method and by electron microscopy. The changes in catecholamine fluorescence were investigated after decentralization, lumbar sympathectomy, and femoral nerve ligation. Catecholamine fluorescence was mainly localized in the arteries, and arterioles in the perimysium of the skeletal muscle. Sometimes, a spot-shaped catecholamine was seen in the metarteriole and capillary. Adrenergic nerve fibers ran parallel with femoral nerve fibers. After lumbar sympathectomy catecholamine fluorescence disappeared, the fluorescence in the quadriceps femoris muscle did not disappear even after ligation of the femoral nerve. Unmyelinated nerve fibers were identified adjacent to the capillary, whereas adrenergic nerve fibers were seen approaching to the quadriceps femoris muscle within a gap of 300 nm.
采用乙醛酸荧光法和电子显微镜研究了大鼠血管、神经和骨骼肌中肾上腺素能神经纤维的分布。在去神经支配、腰交感神经切除和股神经结扎后，对儿茶酚胺荧光的变化进行了研究。儿茶酚胺荧光主要定位于骨骼肌肌束膜中的动脉和小动脉。有时，在微动脉和毛细血管中可见点状儿茶酚胺。肾上腺素能神经纤维与股神经纤维平行走行。腰交感神经切除后儿茶酚胺荧光消失，即使股神经结扎后股四头肌中的荧光也未消失。在毛细血管附近可识别出无髓神经纤维，而在距股四头肌300 nm的间隙内可见肾上腺素能神经纤维向其靠近。
