Ludwicka A, Trojanowska M, Smith E A, Baumann M, Strange C, Korn J H, Smith T, Leroy E C, Silver R M
Department of Medicine, Medical University of South Carolina, Charleston 29425.
J Rheumatol. 1992 Nov;19(11):1716-23.
We describe the detection and the growth of fibroblasts with human smooth muscle cell differentiation features from bronchoalveolar lavage (BAL) fluid of 53% of patients with scleroderma, but not from healthy controls. The binding of alpha-actin, vimentin and desmin antibodies by scleroderma lung fibroblasts exceeded that of normal adult lung fibroblasts, indicating that scleroderma lung fibroblasts express some markers of human smooth muscle cell differentiation (myofibroblasts), which may account for differences in biological behavior. A mesenchymal cell phenotype was documented by mRNA analysis, showing high expression of collagen type I and fibronectin in these cells. Fibronectin is also released in significantly higher amounts by scleroderma alveolar macrophages than by macrophages from healthy donors.
我们描述了在53%的硬皮病患者支气管肺泡灌洗（BAL）液中检测到具有人平滑肌细胞分化特征的成纤维细胞及其生长情况，但在健康对照中未检测到。硬皮病肺成纤维细胞对α - 肌动蛋白、波形蛋白和结蛋白抗体的结合超过了正常成人肺成纤维细胞，这表明硬皮病肺成纤维细胞表达了一些人平滑肌细胞分化的标志物（肌成纤维细胞），这可能解释了生物学行为上的差异。通过mRNA分析证实了间充质细胞表型，显示这些细胞中I型胶原蛋白和纤连蛋白的高表达。硬皮病肺泡巨噬细胞释放的纤连蛋白量也明显高于健康供体的巨噬细胞。
