Swan A J, Heywood B R, Dieppe P A
Rheumatology Unit, Bristol Royal Infirmary, UK.
J Rheumatol. 1992 Nov;19(11):1764-73.
A protocol for extracting minerals from biological tissues and fluids has been developed. Synovial fluid (SF) and articular cartilage samples were treated with papain and sodium hypochlorite to extract and concentrate calcium containing crystals. The method was validated by testing the ability to reextract synthetic calcium pyrophosphate dihydrate and calcium hydroxyapatite crystals added to SF in an unchanged form. The extracted crystals were analyzed by infrared spectroscopy and X-ray powder diffraction. The concentration of both calcium pyrophosphate dihydrate and hydroxyapatite crystals in SF was found to be higher than published figures.
已经开发出一种从生物组织和体液中提取矿物质的方案。用木瓜蛋白酶和次氯酸钠处理滑液（SF）和关节软骨样本，以提取和浓缩含钙晶体。通过测试重新提取添加到SF中的合成二水合焦磷酸钙和羟基磷灰石晶体且使其形式不变的能力，对该方法进行了验证。通过红外光谱和X射线粉末衍射对提取的晶体进行了分析。发现SF中二水合焦磷酸钙和羟基磷灰石晶体的浓度均高于已发表的数据。
