Crocker P R, Dieppe P A, Tyler G, Chapman S K, Willoughby D A
J Pathol. 1977 Jan;121(1):37-40. doi: 10.1002/path.1711210106.
Sections, 1-2 micron thick, of Araldite embedded tissue were prepared to provide a link between optical and electron microscopy for the identification of crystals. This technique permits examination of individual particles by means of polarising light microscopy, electron microscopy, X-ray energy spectroscopy and electron diffraction. It provides a reliable method for routine and research studies. It has led to the positive identification of individual crystals of calcium pyrophosphate dihydrate and hydroxyapatite in synovial fluids. The method can be applied to the identification of other particulate matter in a variety of biological specimens.
制备了厚度为1 - 2微米的用环氧树脂包埋组织的切片，以在光学显微镜和电子显微镜之间建立联系，用于晶体鉴定。该技术允许通过偏光显微镜、电子显微镜、X射线能谱和电子衍射检查单个颗粒。它为常规研究和研究性研究提供了一种可靠的方法。它已导致在滑液中对二水焦磷酸钙和羟基磷灰石的单个晶体进行了阳性鉴定。该方法可应用于鉴定各种生物标本中的其他颗粒物。
