Rasmussen Busund L T, Konopski Z, Oian P, Seljelid R
Institute of Medical Biology, University of Tromsø, Norway.
Microbiol Immunol. 1992;36(11):1173-88. doi: 10.1111/j.1348-0421.1992.tb02120.x.
Human monocytes, human peritoneal macrophages, mouse peritoneal macrophages and human peripheral neutrophils pretreated with beta-1,3-D-polyglucose derivatives showed pronounced bactericidal capacity to Escherichia coli compared to control cells. The increased bactericidal capacity was detectable in mononuclear phagocytes over a wide range of concentrations of bacteria. Granulocytes, however, showed bactericidal capacity only at low concentrations of bacteria. The pretreated mononuclear phagocytes released significant amounts of IL-1 and PGE2. However, there was no significant release of tumor necrosis factor (TNF). By incubating unstimulated cells with purified IL-1 and TNF, the bactericidal activity of neutrophils and mononuclear phagocytes was enhanced. Our data indicate that the inability of neutrophils stimulated with beta-1,3-D-polyglucose derivatives to kill large numbers of bacteria could be overcome by a combined treatment with purified IL-1 or TNF in addition to beta-1,3-D-polyglucose derivatives. By incubating unstimulated cells with medium from beta-1,3-D-polyglucose-treated human peritoneal macrophages, the bactericidal activity of the cells was enhanced to the same extent as cells pretreated with purified TNF and IL-1. Cells incubated with IL-1-depleted medium from beta-1,3-D-polyglucose-treated human peritoneal macrophages, showed reduced bactericidal activity compared to cells incubated with undepleted medium. These studies demonstrate that beta-1,3-D-polyglucose-treated mononuclear phagocytes and neutrophils show enhanced bactericidal activity. The enhanced activity is partly caused by stimulation of the cells with IL-1 released from mononuclear phagocytes and partly by other unknown effects of beta-1,3-D-polyglucose derivatives on both mononuclear phagocytes and neutrophils.
与对照细胞相比，用β-1,3-D-聚葡萄糖衍生物预处理的人单核细胞、人腹腔巨噬细胞、小鼠腹腔巨噬细胞和人外周血中性粒细胞对大肠杆菌显示出显著的杀菌能力。在广泛的细菌浓度范围内，单核吞噬细胞的杀菌能力增强是可检测到的。然而，粒细胞仅在低细菌浓度下显示杀菌能力。预处理的单核吞噬细胞释放大量的白细胞介素-1（IL-1）和前列腺素E2（PGE2）。然而，肿瘤坏死因子（TNF）没有显著释放。通过用纯化的IL-1和TNF孵育未刺激的细胞，中性粒细胞和单核吞噬细胞的杀菌活性增强。我们的数据表明，用β-1,3-D-聚葡萄糖衍生物刺激的中性粒细胞无法杀死大量细菌的情况，可以通过联合使用纯化的IL-1或TNF以及β-1,3-D-聚葡萄糖衍生物来克服。通过用β-1,3-D-聚葡萄糖处理的人腹腔巨噬细胞的培养基孵育未刺激的细胞，细胞的杀菌活性增强到与用纯化的TNF和IL-1预处理的细胞相同的程度。与用未耗尽培养基孵育的细胞相比，用β-1,3-D-聚葡萄糖处理的人腹腔巨噬细胞的IL-1耗尽培养基孵育的细胞显示出降低的杀菌活性。这些研究表明，β-1,3-D-聚葡萄糖处理的单核吞噬细胞和中性粒细胞显示出增强的杀菌活性。这种增强的活性部分是由单核吞噬细胞释放的IL-1刺激细胞引起的，部分是由β-1,3-D-聚葡萄糖衍生物对单核吞噬细胞和中性粒细胞的其他未知作用引起的。
