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大肠杆菌OxyR对dam+细胞中噬菌体Mu mom表达的调控可归因于其结合半甲基化Pmom启动子DNA的能力。

Escherichia coli OxyR modulation of bacteriophage Mu mom expression in dam+ cells can be attributed to its ability to bind hemimethylated Pmom promoter DNA.

作者信息

Hattman S, Sun W

机构信息

Department of Biology, University of Rochester, Rochester, NY 14627, USA.

出版信息

Nucleic Acids Res. 1997 Nov 1;25(21):4385-8. doi: 10.1093/nar/25.21.4385.

DOI:10.1093/nar/25.21.4385
PMID:9336472
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC147061/
Abstract

Transcription of the bacteriophage Mu mom operon is strongly repressed by the host OxyR protein in dam - but not dam + cells. In this work we show that the extent of mom modification is sensitive to the relative levels of the Dam and OxyR proteins and OxyR appears to modulate the level of mom expression even in dam + cells. In vitro studies demonstrated that OxyR is capable of binding hemimethylated P mom , although its affinity is reduced slightly compared with unmethylated DNA. Thus, OxyR modulation of mom expression in dam + cells can be attributed to its ability to bind hemimethylated P mom DNA, the product of DNA replication.

摘要

噬菌体Mu mom操纵子的转录在dam - 细胞而非dam + 细胞中受到宿主OxyR蛋白的强烈抑制。在本研究中，我们发现mom修饰的程度对Dam和OxyR蛋白的相对水平敏感，并且即使在dam + 细胞中，OxyR似乎也能调节mom的表达水平。体外研究表明，OxyR能够结合半甲基化的P mom ，尽管与未甲基化的DNA相比，其亲和力略有降低。因此，OxyR在dam + 细胞中对mom表达的调节可归因于其结合DNA复制产物半甲基化P mom DNA的能力。

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1
Escherichia coli OxyR modulation of bacteriophage Mu mom expression in dam+ cells can be attributed to its ability to bind hemimethylated Pmom promoter DNA.大肠杆菌OxyR对dam+细胞中噬菌体Mu mom表达的调控可归因于其结合半甲基化Pmom启动子DNA的能力。
Nucleic Acids Res. 1997 Nov 1;25(21):4385-8. doi: 10.1093/nar/25.21.4385.
2
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本文引用的文献

1
Escherichia coli OxyR protein represses the unmethylated bacteriophage Mu mom operon without blocking binding of the transcriptional activator C.大肠杆菌OxyR蛋白抑制未甲基化的噬菌体Mu mom操纵子，而不阻止转录激活因子C的结合。
Nucleic Acids Res. 1996 Oct 15;24(20):4042-9. doi: 10.1093/nar/24.20.4042.
2
In vitro transcriptional activation of the phage Mu mom promoter by C protein.C蛋白对噬菌体Mu mom启动子的体外转录激活作用。
J Bacteriol. 1994 May;176(10):2885-91. doi: 10.1128/jb.176.10.2885-2891.1994.
3
Redox-dependent shift of OxyR-DNA contacts along an extended DNA-binding site: a mechanism for differential promoter selection.OxyR与DNA的结合沿扩展的DNA结合位点发生氧化还原依赖性移位：一种差异启动子选择机制。
Cell. 1994 Sep 9;78(5):897-909. doi: 10.1016/s0092-8674(94)90702-1.
4
Mutational analysis of the redox-sensitive transcriptional regulator OxyR: regions important for DNA binding and multimerization.氧化还原敏感转录调节因子OxyR的突变分析：对DNA结合和多聚化重要的区域
J Bacteriol. 1995 Mar;177(5):1285-91. doi: 10.1128/jb.177.5.1285-1291.1995.
5
DNA methyltransferase-dependent transcription of the phage Mu mom gene.噬菌体Mu mom基因的DNA甲基转移酶依赖性转录
Proc Natl Acad Sci U S A. 1982 Sep;79(18):5518-21. doi: 10.1073/pnas.79.18.5518.
6
Purification and characterization of the unusual deoxynucleoside, alpha-N-(9-beta-D-2'-deoxyribofuranosylpurin-6-yl)glycinamide, specified by the phage Mu modification function.由噬菌体Mu修饰功能所指定的异常脱氧核苷α-N-(9-β-D-2'-脱氧呋喃核糖基嘌呤-6-基)甘氨酰胺的纯化与特性鉴定
Proc Natl Acad Sci U S A. 1983 Dec;80(24):7400-4. doi: 10.1073/pnas.80.24.7400.
7
Methylation regulates the expression of a DNA-modification function encoded by bacteriophage Mu.甲基化调控由噬菌体Mu编码的一种DNA修饰功能的表达。
Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:639-46. doi: 10.1101/sqb.1983.047.01.075.
8
Regulation of the DNA-modification function of bacteriophage Mu.噬菌体Mu的DNA修饰功能调控
Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:647-53. doi: 10.1101/sqb.1983.047.01.076.
9
The mom gene of bacteriophage Mu.噬菌体Mu的mom基因。
Curr Top Microbiol Immunol. 1984;108:29-47. doi: 10.1007/978-3-642-69370-0_4.
10
Transcription initiation of Mu mom depends on methylation of the promoter region and a phage-coded transactivator.Mu噬菌体妈妈基因的转录起始依赖于启动子区域的甲基化作用以及一种噬菌体编码的反式激活因子。
Nature. 1983 Jan 27;301(5898):344-7. doi: 10.1038/301344a0.