Yamashita T, Tanaka T, Warashina M, Kondo K, Goto Y, Kawai S, Kurai K, Kunito S, Monna T, Matsui T
Department of Public Health, Osaka City University Medical School, Japan.
Arukoru Kenkyuto Yakubutsu Ison. 1992 Dec;27(6):613-22.
This investigation was performed to determine whether acute ethanol administration (5 g and 3 g/kg body wt.) alters the lipid composition both of liver plasma membrane (LPM) and serum in rat or not. The changes of lipid composition of LPM and serum were observed during peak values which blood ethanol concentration was achieved, after both 5 g and 3 g/kg body wt. ethanol administration; LPM cholesterol (CH) content was significantly decreased, but LPM phospholipids (PL) content was not changed statistically. Serum CH and PL contents were significantly increased. However, CH/PL molar ratio showed significant decreases in both LPM and serum. SPM/PC in LPM was also decreased significantly. These changes of lipid composition in LPM were associated with the increase of the fluidity as demonstrated in our previous reports. These lipid values in ethanol treated animals expressed as a ratio of those from corresponding control animals, taken as 1, consisted with 0.7-0.8, that is very interesting from viewpoint of "homeoviscous adaptation", and in addition, suggesting the possibility that serum CH/PL molar ratio may be useful for the estimation of the changes of lipid composition concerned with LPM fluidity.
本研究旨在确定急性给予乙醇（5克/千克体重和3克/千克体重）是否会改变大鼠肝细胞膜（LPM）和血清中的脂质成分。在给予5克/千克体重和3克/千克体重乙醇后，在达到血乙醇浓度峰值期间观察LPM和血清脂质成分的变化；LPM胆固醇（CH）含量显著降低，但LPM磷脂（PL）含量无统计学变化。血清CH和PL含量显著增加。然而，CH/PL摩尔比在LPM和血清中均显著降低。LPM中的鞘磷脂/磷脂酰胆碱（SPM/PC）也显著降低。如我们之前的报告所示，LPM中脂质成分的这些变化与流动性增加有关。乙醇处理动物的这些脂质值表示为相应对照动物的脂质值之比（设为1），为0.7 - 0.8，从“等膜粘度适应”的角度来看非常有趣，此外，这表明血清CH/PL摩尔比可能有助于评估与LPM流动性相关的脂质成分变化。
