使用pH指示剂测量冷冻缓冲溶液的pH值。
Measurement of the pH of frozen buffer solutions by using pH indicators.
作者信息
Orii Y, Morita M
出版信息
J Biochem. 1977 Jan;81(1):163-8. doi: 10.1093/oxfordjournals.jbchem.a131431.
Abstract
A method was established to estimate the pH change of several buffers solutions on freezing by using a combination of pH indicators. Among more than 30 buffers solutions examined, almost half exhibited a pH change in the temperature range between freezing point and 220 degrees K; the results were tabulated. Glycerol was found to suppress the pH changes because of its "salt buffer" effect.
摘要
建立了一种通过使用pH指示剂组合来估算几种缓冲溶液冷冻时pH变化的方法。在所研究的30多种缓冲溶液中，几乎一半在冰点至220开尔文的温度范围内表现出pH变化；结果已列表。由于甘油的“盐缓冲”作用，发现它能抑制pH变化。
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