未包被质粒DNA的原子力显微镜观察：仅需纳克量样品即可实现纳米级分辨率

Atomic force microscopy of uncoated plasmid DNA: nanometer resolution with only nanogram amounts of sample.

作者信息

Li M Q, Hansma H G, Vesenka J, Kelderman G, Hansma P K

机构信息

Department of Physics, University of California, Santa Barbara 93106.

出版信息

J Biomol Struct Dyn. 1992 Dec;10(3):607-17. doi: 10.1080/07391102.1992.10508671.

DOI:10.1080/07391102.1992.10508671

PMID:1492927

Abstract

Reproducible, high-contrast, nanometer-resolution AFM images of uncoated plasmid DNA can be obtained with nanogram quantities of DNA with the help of two advances in sample preparation: (1) Heating a DNA solution at 35 degrees C for 10 to 20 minutes before deposition on mica helps separate and spread the DNA, and (2) Using 5 microliter drops of the heated DNA solution in the concentration range of 2 to 10 nanogram/microliter in contact with a specially prepared mica surface for 5 to 10 minutes gives optimal coverage with only nanograms of DNA.

摘要

借助样品制备方面的两项进展，使用纳克级量的未包被质粒DNA即可获得具有可重复性、高对比度、纳米分辨率的原子力显微镜（AFM）图像：（1）在将DNA溶液沉积到云母上之前，于35摄氏度加热10至20分钟，这有助于DNA分离和铺展；（2）将浓度在2至10纳克/微升范围内的5微升加热后的DNA溶液滴在经过特殊处理的云母表面上，接触5至10分钟，仅用纳克级的DNA就能实现最佳覆盖。