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通过原子力显微镜观察细菌多糖乙酰聚糖的螺旋结构。

Observation of the helical structure of the bacterial polysaccharide acetan by atomic force microscopy.

作者信息

Kirby A R, Gunning A P, Morris V J, Ridout M J

机构信息

Institute of Food Research, Colney, Norwich, United Kingdom.

出版信息

Biophys J. 1995 Jan;68(1):360-3. doi: 10.1016/S0006-3495(95)80195-5.

DOI:10.1016/S0006-3495(95)80195-5
PMID:7711262
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1281695/
Abstract

A method has been developed that has been found to give reproducible images of uncoated polysaccharides by Atomic Force Microscopy (AFM). Aqueous solutions of the polysaccharide are deposited as drops onto freshly cleaved mica surfaces, air dried, and then imaged under butanol. The method has been used to obtain images of the bacterial polysaccharide acetan. In regions within the deposited sample, where the molecules are aligned side-by-side, it has been possible to observe a periodic structure along the polysaccharide chain, attributable to the helical structure of acetan.

摘要

已开发出一种方法，通过原子力显微镜（AFM）可得到未涂层多糖的可重复图像。将多糖水溶液以液滴形式沉积在新劈开的云母表面上，空气干燥，然后在丁醇下成像。该方法已用于获取细菌多糖乙酰聚糖的图像。在沉积样品内分子并排排列的区域，可以观察到沿多糖链的周期性结构，这归因于乙酰聚糖的螺旋结构。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/998d/1281695/9ace0514abb8/biophysj00067-0364-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/998d/1281695/f3b8b668bfba/biophysj00067-0363-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/998d/1281695/e040dbee45ff/biophysj00067-0364-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/998d/1281695/9ace0514abb8/biophysj00067-0364-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/998d/1281695/f3b8b668bfba/biophysj00067-0363-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/998d/1281695/e040dbee45ff/biophysj00067-0364-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/998d/1281695/9ace0514abb8/biophysj00067-0364-b.jpg

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