Bucala R
Laboratory of Medical Biochemistry, Rockefeller University, New York.
Immunology. 1992 Dec;77(4):477-82.
Lipopolysaccharide (LPS) is a potent murine polyclonal B-cell activator which induces cellular proliferation and IgM secretion. The precise role of activated macrophages in the induction of LPS-dependent, B-cell responses has been unclear. Although early reports concluded that the LPS effect occurs independently of other cell types, other studies have suggested that adherent macrophages exert either potentiating or inhibitory effects. In the present study, B-cell mitogenesis and IgM production were measured in primary spleen cell cultures after removing adherent cells by a variety of experimental procedures. B-cell activation by LPS was found to be strictly dependent on the presence of adherent macrophages. Antibody neutralization and cytokine reconstitution studies demonstrated that macrophage-derived interleukin- (IL-1) is a necessary co-factor for LPS-induced polyclonal activation.
脂多糖（LPS）是一种强效的小鼠多克隆B细胞激活剂，可诱导细胞增殖和IgM分泌。活化的巨噬细胞在LPS依赖性B细胞反应诱导中的精确作用尚不清楚。尽管早期报告得出结论，LPS的作用独立于其他细胞类型发生，但其他研究表明，贴壁巨噬细胞发挥增强或抑制作用。在本研究中，通过各种实验程序去除贴壁细胞后，在原代脾细胞培养物中测量B细胞有丝分裂和IgM产生。发现LPS对B细胞的激活严格依赖于贴壁巨噬细胞的存在。抗体中和和细胞因子重组研究表明，巨噬细胞衍生的白细胞介素-1（IL-1）是LPS诱导的多克隆激活所必需的辅助因子。
