Fernandez C, Severinson E
Scand J Immunol. 1983 Oct;18(4):279-89. doi: 10.1111/j.1365-3083.1983.tb01799.x.
Different concentrations of spleen cells from C57BL.10 mice were activated with lipopolysaccharide (LPS), and DNA synthesis and IgM and IgG secretion were measured. The dilution curve was sigmoid, and the response was rapidly lost below a certain cell concentration. In the presence of thymocytes or spleen cells from the LPS-non-responder strain C57BL.10/ScCr the dilution curve for the LPS response of cells from C57BL.10 mice became linear, and the overall response was increased. Irradiated cells could not restore the response at suboptimal cell concentrations. In addition, enriched T cells restored the response as well as normal spleen cells, whereas enriched B cells did not. We compared the restoring capacity of different filler cells for the LPS response with that of a plasmacytoma cell line cultured at suboptimal cell concentrations. The results are compatible with the idea that the filler cells provide growth-stimulating activity to LPS-responsive cells but growth-supporting activity to the tumour cells. Furthermore, highly enriched B-cell populations respond poorly to LPS, but the response can be partly restored by filler cells. These data suggest that the LPS response is accessory-cell-dependent.
用脂多糖（LPS）激活来自C57BL.10小鼠的不同浓度的脾细胞，并检测DNA合成以及IgM和IgG分泌。稀释曲线呈S形，且在低于一定细胞浓度时反应迅速消失。在存在来自LPS无反应株C57BL.10/ScCr的胸腺细胞或脾细胞的情况下，C57BL.10小鼠细胞对LPS反应的稀释曲线变为线性，且总体反应增强。经辐照的细胞在亚最佳细胞浓度下无法恢复反应。此外，富集的T细胞与正常脾细胞一样能恢复反应，而富集的B细胞则不能。我们将不同填充细胞对LPS反应的恢复能力与在亚最佳细胞浓度下培养的浆细胞瘤细胞系的恢复能力进行了比较。结果与以下观点相符，即填充细胞为LPS反应性细胞提供生长刺激活性，但为肿瘤细胞提供生长支持活性。此外，高度富集的B细胞群体对LPS反应较差，但填充细胞可部分恢复该反应。这些数据表明LPS反应依赖于辅助细胞。
