Shieh E, Moreira H, D'Arcy J, Clapham T N, McDonnell P J
Doheny Eye Institute, Los Angeles, CA 90033.
Ophthalmology. 1992 Jul;99(7):1050-5. doi: 10.1016/s0161-6420(92)31851-2.
Superficial corneal ablation using the 193 nm excimer laser for the correction of myopia and astigmatism produces histologic changes in the corneal profile that can be quantitatively analyzed.
Using the excimer laser, spherical and cylindrical corneal ablations were performed on rabbit eyes at depths of 2, 4, 6, and 8 diopters. After 12 weeks, the corneas were examined histologically using a dichlorotriazinyl aminofluorescein stain for collagen, and the results were quantified using a digital video image analysis system.
Epithelial thickness and thickness of new collagen were positively correlated with the depth of ablation after spherical ablations. With astigmatic ablations, epithelial thickness and new collagen production were correlated with ablation depth in one axis only.
These results suggest that during cylindrical corneal ablations with the excimer laser, radially asymmetrical stromal ablations produce quantitatively asymmetrical wound healing.
使用193纳米准分子激光进行角膜表面消融以矫正近视和散光，会在角膜轮廓上产生可进行定量分析的组织学变化。
使用准分子激光，对兔眼进行2、4、6和8屈光度深度的球面和柱面角膜消融。12周后，使用二氯三嗪基氨基荧光素对胶原蛋白进行组织学检查，并使用数字视频图像分析系统对结果进行量化。
球面消融后，上皮厚度和新胶原蛋白厚度与消融深度呈正相关。对于散光消融，上皮厚度和新胶原蛋白生成仅与一个轴上的消融深度相关。
这些结果表明，在使用准分子激光进行柱面角膜消融时，径向不对称的基质消融会产生定量不对称的伤口愈合。
