Dusart G, Zuccarelli M, Ossia-Ongagna Y, Simeon de Buochberg M
Faculté de Pharmacie, Laboratoire de Bactériologie et de contrôle Microbiologique, Montpellier.
Pathol Biol (Paris). 1992 May;40(5):523-8.
The bactericidal and sporicidal activities of a peracetic acid disinfectant commonly used in hospitals (Acetoper 200) was studied using three bacterial species recovered in water from hemodialysis machines and humidifiers, i.e., Pseudomonas aeruginosa, Serratia liquefaciens, and Bacillus subtilis (spores). The method used was derived from AFNOR norms NF T 72-151 and NF T 72-231. For each strain, three concentrations of disinfectant were tested. Counts were performed every five minutes for one hour to evaluate killing kinetics. For both Gram-negative organisms, survival curves were biphasic, whereas B. subtilis counts decreased logarithmically. Both concentration and time influenced the 5 log10 decrease in counts. With S. liquefaciens and B. subtilis (spores), the 5 log10 decrease was not reached with low levels of disinfectant. In every case, the D value decreased substantially with increasing levels of disinfectant.
使用从血液透析机和加湿器的水中分离出的三种细菌，即铜绿假单胞菌、液化沙雷氏菌和枯草芽孢杆菌（芽孢），对医院常用的一种过氧乙酸消毒剂（Acetoper 200）的杀菌和杀芽孢活性进行了研究。所采用的方法源自法国标准化协会（AFNOR）标准NF T 72 - 151和NF T 72 - 231。对每种菌株测试了三种消毒剂浓度。每隔五分钟计数一次，持续一小时以评估杀灭动力学。对于两种革兰氏阴性菌，存活曲线呈双相，而枯草芽孢杆菌的菌数呈对数下降。浓度和时间均影响菌数下降5个对数级。对于液化沙雷氏菌和枯草芽孢杆菌（芽孢），低水平消毒剂无法使菌数下降5个对数级。在每种情况下，随着消毒剂水平的提高，D值大幅下降。
