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[利用酯酶电泳多态性分析和抗生素耐药谱分析对产碱杆菌属菌种进行鉴定]

[Characterization of Alcaligenes species using analysis of esterase electrophoretic polymorphism and analysis of antibiotic resistance profiles].

作者信息

Bizet C, Picard B, Philippon A, Goullet P

机构信息

Institut Pasteur, Service de la Collection, Paris.

出版信息

Pathol Biol (Paris). 1992 May;40(5):591-8.

PMID:1495848
Abstract

The species of an Alcaligenes bacterial strain may be difficult to determine on the basis of conventional phenotype features. Esterase pattern analysis using acrylamide-agar gel electrophoresis and determination of the antimicrobial resistance profile (agar diffusion method) were performed for A. faecalis (34 strains). A. denitrificans subsp xylosoxydans (16 strains) and A. piechaudi (5 strains). The Cistat program (D2 Software) was used for statistical representation of results. The homogeneous, species-specific esterase patterns ensured correct assignment of each strain to one of the three species. Antimicrobial susceptibility was greatest for A. faecalis which was susceptible to both cephalosporins of all generations and aminoglycosides. A. xylosoxydans was the species with the greatest resistance to antimicrobials. A. piechaudii exhibited intermediate susceptibility.

摘要

基于传统的表型特征可能难以确定产碱菌菌株的种类。对粪产碱菌(34株)、反硝化产碱菌木糖氧化亚种(16株)和皮氏产碱菌(5株)进行了使用丙烯酰胺 - 琼脂凝胶电泳的酯酶图谱分析以及抗菌药物敏感性分析(琼脂扩散法)。结果的统计表示使用了Cistat程序(D2软件)。均一的、物种特异性的酯酶图谱确保了每株菌能正确归为这三个物种之一。粪产碱菌的抗菌敏感性最高,对所有代的头孢菌素和氨基糖苷类药物均敏感。木糖氧化产碱菌是对抗菌药物耐药性最强的物种。皮氏产碱菌表现出中等敏感性。

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