Tachibana H, Kobayashi S, Paz K C, Aca I S, Tateno S, Ihara S
Laboratório de Imunopatologia Prof. Keizo Asami, Universidade Federal de Pernambuco, Recife, Brazil.
Parasitol Res. 1992;78(5):433-6. doi: 10.1007/BF00931701.
The pathogenicity of 47 strains of Entamoeba histolytica isolated in Pernambuco, Brazil, was examined using the polymerase chain reaction (PCR) followed by restriction-endonuclease digestion. Electrophoretic patterns of PCR products digested with HinfI revealed that all strains were nonpathogenic. The results were entirely in accord with phenotypic properties such as isoenzyme patterns and the failure to bind a pathogenic-isolate-specific monoclonal antibody. When the sensitivity of PCR was examined, amplified products could be detected from template DNA equivalent to five trophozoites. These observations indicate that PCR amplification of genomic DNA and subsequent restriction-enzyme digestion is a useful strategy for obtaining a sensitive and accurate diagnosis. The present study also demonstrates that nonpathogenic strains of E. histolytica predominate in northeastern Brazil.
利用聚合酶链反应（PCR），随后进行限制性内切酶消化，对在巴西伯南布哥州分离出的47株溶组织内阿米巴的致病性进行了检测。用HinfI消化的PCR产物的电泳图谱显示，所有菌株均无致病性。结果与同工酶谱等表型特性以及未能结合致病性分离株特异性单克隆抗体完全一致。当检测PCR的灵敏度时，从相当于五个滋养体的模板DNA中可检测到扩增产物。这些观察结果表明，基因组DNA的PCR扩增及随后的限制性酶切消化是获得灵敏且准确诊断的有用策略。本研究还表明，非致病性溶组织内阿米巴菌株在巴西东北部占主导地位。
