Häusler A, Ballou L, Ballou C E, Robbins P W
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):6846-50. doi: 10.1073/pnas.89.15.6846.
The Saccharomyces cerevisiae MNT1 gene encodes a Golgi mannosyltransferase. Gene disruption of the MNT1 locus leads to a greater than 90% reduction of specific alpha-1,2-mannosyltransferase activity with alpha-methylmannoside as acceptor. Null mutants of MNT1 are viable, have no apparent growth defect, and are blocked in the elongation of protein O-linked mannobiose. Structural analysis of the N-linked outer chain isolated from an mnn1 mnn10 mnt1 strain revealed no alteration in carbohydrate structure compared to the parental mnn1 mnn10 strain. The MNT1 gene is identical to KRE2, and mutations in the gene render cells resistant to the killer toxin K1 of S. cerevisiae, which suggests a role for O-mannosylated proteins in the resistance mechanism. In addition, MNT1 is part of a multigene family whose members are presumed to be yeast Golgi mannosyltransferases.
酿酒酵母MNT1基因编码一种高尔基体甘露糖基转移酶。MNT1基因座的基因破坏导致以α-甲基甘露糖苷为受体的特定α-1,2-甘露糖基转移酶活性降低90%以上。MNT1的缺失突变体是有活力的，没有明显的生长缺陷，并且在蛋白质O-连接甘露二糖的延伸过程中受阻。从mnn1 mnn10 mnt1菌株中分离出的N-连接外链的结构分析表明，与亲本mnn1 mnn10菌株相比，碳水化合物结构没有改变。MNT1基因与KRE2相同，该基因中的突变使细胞对酿酒酵母的杀伤毒素K1具有抗性，这表明O-甘露糖基化蛋白在抗性机制中起作用。此外，MNT1是一个多基因家族的成员，其成员被认为是酵母高尔基体甘露糖基转移酶。
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