濒死期和死后因素对基因表达谱的影响：人死后大脑微阵列分析中的质量控制
Effect of agonal and postmortem factors on gene expression profile: quality control in microarray analyses of postmortem human brain.
作者信息
Tomita Hiroaki, Vawter Marquis P, Walsh David M, Evans Simon J, Choudary Prabhakara V, Li Jun, Overman Kevin M, Atz Mary E, Myers Richard M, Jones Edward G, Watson Stanley J, Akil Huda, Bunney William E
机构信息
Department of Psychiatry and Human Behavior, University of California-Irvine, Irvine, CA 92697-1675, USA.
出版信息
Biol Psychiatry. 2004 Feb 15;55(4):346-52. doi: 10.1016/j.biopsych.2003.10.013.
Abstract
There are major concerns that specific agonal conditions, including coma and hypoxia, might affect ribonucleic acid (RNA) integrity in postmortem brain studies. We report that agonal factors significantly affect RNA integrity and have a major impact on gene expression profiles in microarrays. In contrast to agonal factors, gender, age, and postmortem factors have less effect on gene expression profiles. The Average Correlation Index is proposed as a method for evaluating RNA integrity on the basis of similarity of microarray profiles. Reducing the variance due to agonal factors is critical in investigating small but validated gene expression differences in messenger RNA levels between psychiatric patients and control subjects.
摘要
人们主要担心，包括昏迷和缺氧在内的特定濒死状态可能会影响死后大脑研究中核糖核酸（RNA）的完整性。我们报告称，濒死因素会显著影响RNA完整性，并对微阵列中的基因表达谱产生重大影响。与濒死因素相比，性别、年龄和死后因素对基因表达谱的影响较小。提出了平均相关指数作为一种基于微阵列谱相似性评估RNA完整性的方法。在研究精神病患者和对照受试者之间信使RNA水平上微小但经过验证的基因表达差异时，减少濒死因素导致的方差至关重要。
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