Kodzius Rimantas, Matsumura Yonehiro, Kasukawa Takeya, Shimokawa Kazuro, Fukuda Shiro, Shiraki Toshiyuki, Nakamura Mari, Arakawa Takahiro, Sasaki Daisuke, Kawai Jun, Harbers Matthias, Carninci Piero, Hayashizaki Yoshihide
Genome Science Laboratory, RIKEN, Wako Main Campus, Hirosawa 2-1, Wako, Saitama 351-0198, Japan.
FEBS Lett. 2004 Feb 13;559(1-3):22-6. doi: 10.1016/S0014-5793(04)00018-3.
The RIKEN expression array database (READ) provides comprehensive gene expression data for the mouse, which were obtained as relative values from microarray double-staining experiments with E17.5 mRNA as common reference. To assign absolute expression values for mouse transcripts within READ, we applied the E17.5 reference sample to CAGE (cap analysis of gene expression) and expressed sequence tag (EST) high-throughput tag sequencing. Newly assigned values within the READ database were validated by comparison to expression data from serial analysis of gene expression, CAGE and EST experiments. These experiments confirmed the great significance of the absolute expression values within the improved READ database. The new Absolute READ database on absolute expression data is available under.
日本理化学研究所表达阵列数据库（READ）提供了小鼠的全面基因表达数据，这些数据是通过以E17.5 mRNA作为共同参考的微阵列双重染色实验获得的相对值。为了在READ中为小鼠转录本分配绝对表达值，我们将E17.5参考样本应用于基因表达的帽分析（CAGE）和表达序列标签（EST）高通量标签测序。通过与基因表达的序列分析、CAGE和EST实验的表达数据进行比较，验证了READ数据库中新分配的值。这些实验证实了改进后的READ数据库中绝对表达值的重要意义。关于绝对表达数据的新的绝对READ数据库可在以下网址获取。
